Microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; an in vitro and in vivo study
이효정, Lee Jaden, 이정태, 홍지수, 임범순, 박희정, 김영광, 김태일,
소속 상세정보
이효정 ( Lee Hyo-Jung ) - Seoul National University Bundang Hospital Section of Dentistry Department of Periodontology
( Lee Jaden ) - Medical University of South Carolina College of Dental Medicine Department of Oral Health Sciences
이정태 ( Lee Jung-Tae ) - Seoul National University Bundang Hospital Section of Dentistry Department of Periodontology
홍지수 ( Hong Ji-Soo ) - Seoul National University School of Dentistry Department of Oral Pathology
임범순 ( Lim Bum-Soon ) - Seoul National University School of Dentistry Department of Dental Biomaterials
박희정 ( Park Hee-Jung ) - Korea University Department of Public Health Sciences
김영광 ( Kim Young-Kwang ) - Boston University School of Dental Medicine Department of General Dentistry
김태일 ( Kim Tae-Il ) - Seoul National University School of Dentistry Department of Periodontology
KMID : 0363020150450030120
Abstract
Purpose: With the significance of stable adhesion of alveolar bone and peri-implant soft tissue on the surface of titanium for successful dental implantation procedure, the purpose of this study was to apply microgrooves on the titanium surface and investigate their effects on peri-implant cells and tissues.
Methods: Three types of commercially pure titanium discs were prepared; machined-surface discs (A), sandblasted, large-grit, acid-etched (SLA)-treated discs (B), SLA and microgroove-formed discs (C). After surface topography of the discs was examined by confocal laser scanning electron microscopy, water contact angle and surface energy were measured. Human gingival fibroblasts (hGFs) and murine osteoblastic cells (MC3T3-E1) were seeded onto the titanium discs for immunofluorescence assay of adhesion proteins. Commercially pure titanium implants with microgrooves on the coronal microthreads design were inserted into the edentulous mandible of beagle dogs. After 2 weeks and 6 weeks of implant insertion, the animal subjects were euthanized to confirm peri-implant tissue healing pattern in histologic specimens.
Results: Group C presented the lowest water contact angle (62.89±5.66 θ), highest surface energy (45±1.2 mN/m), and highest surface roughness (Ra=22.351±2.766 μm). The expression of adhesion molecules of hGFs and MC3T30E1 cells was prominent in group C. Titanium implants with microgrooves on the coronal portion showed firm adhesion to peri-implant soft tissue.
Conclusions: Microgrooves on the titanium surface promoted the adhesion of gingival fibroblasts and osteoblastic cells, as well as favorable peri-implant soft tissue sealing.
키워드
Cell adhesion ; Dental implants ; Titanium ; Wound healing
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